CD40-activated B cells express full lymph node homing triad and induce T-cell chemotaxis: potential as cellular adjuvants.

نویسندگان

  • Michael von Bergwelt-Baildon
  • Alexander Shimabukuro-Vornhagen
  • Alexey Popov
  • Nela Klein-Gonzalez
  • Francesca Fiore
  • Svenja Debey
  • Andreas Draube
  • Britta Maecker
  • Isaura Menezes
  • Lee M Nadler
  • Joachim L Schultze
چکیده

CD40-activated B cells (CD40-B cells) have previously been introduced as an alternative source of antigen-presenting cells for immunotherapy. CD40-B cells can prime naive and expand memory T cells, and they can be generated in large numbers from very small amounts of peripheral blood derived from healthy individuals or cancer patients alike. Administration of CD40-B cells as a cellular adjuvant would require these cells to migrate toward secondary lymphoid organs and attract T cells in situ, processes guided by specific chemokines and chemokine receptors. Here, we demonstrate that primary, human CD40-B cells express a pattern of adhesion molecules and chemokine receptors necessary for homing to secondary lymphoid organs and have the capacity to migrate to cognate ligands. Furthermore, we show that CD40-B cells express important T-cell attractants and induce strong T-cell chemotaxis. These findings further support the use of CD40-B cells as cellular adjuvant for cancer immunotherapy.

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عنوان ژورنال:
  • Blood

دوره 107 7  شماره 

صفحات  -

تاریخ انتشار 2006